Indirect Coombs Test Cost in Hyderabad

Why is the Indirect Coombs test done?

• Before any blood transfusion, it is essential to determine the compatibility between the donor’s blood and the recipient’s blood. So this test helps to identify any antibodies present in the recipient’s blood that may react with the donor’s blood.

• This test is also done on pregnant women to access the risk of  Hemolytic disease in the newborn(HDN), this can be done when the mother’s immune system produces antibodies that attack the red blood cells of the developing fetus. 

• Autoimmune Hemolytic Anemia (AIHA) is a condition where a person’s immune system mistakenly attacks their red blood cells. The indirect Coombs test can help determine the presence of antibodies that are responsible for this destruction.

Why is the Indirect Coombs test done during pregnancy?

An indirect Coombs test is done during pregnancy to reduce the risk of hemolytic disease of the newborn (HDN), also known as erythroblastosis fetalis. HDN occurs when a mother’s immune system produces antibodies that attack the red blood cells of the developing fetus. During the pregnancy, there might be a blood group incompatibility between the mother and the fetus, this usually happens when the mother has Rh-negative and the father has Rh-positive. If the fetus inherits the Rh antigen enters from the father to the mother’s bloodstream during pregnancy or childbirth, the mother’s immune system may produce antibodies against the Rh antigen. The indirect Coombs test helps detect the presence of these antibodies in the mother’s blood.

How is an Indirect Coombs test done?

• Sample collection: A sample is collected from the patient, usually from the vein in the arm.
• Separation of the serum: The collected blood sample is centrifuged to separate the serum, which is the yellowish liquid portion of the blood, from the red blood cells.
• Mixing the patient’s serum:  A small amount of serum is added to each test tube that contains red blood cells. The mixture allows antibodies present in the patient’s serum to react with the antigens on the red blood cells.
• Incubation: The test tubes are incubated at a specific temperature to allow sufficient time for the antibody-antigen reactions to occur.
• Addition of Coombs Reagent: This Coomb Reagent is added to each test tube, The Coombs reagent consists of antibodies that can bind to antibodies present on the patient’s red blood cells, forming visible clumps.
• Observation and interpretation: The test tubes are observed for agglutination.  Agglutination indicates a positive result, meaning that the patient’s serum contains antibodies that have reacted with the red blood cells in the test tube. A negative result indicates the absence of detectable antibodies in the patient’s serum.

Why is Medifyhome the best choice for the test?

Medifyhome is one of the well-known Health services, also associated with the best diagnostic centers in Hyderabad for affordable prices. Medifyhome is also known for its Home Based Services, has top technicians with good qualifications and experience, and has the best equipment. All the Laboratories we network with are NABL accredited and provide accurate and reliable results, Results will be given within 24 hours we will generate the report through online.